The orientation of a real vector space or simply orientation of a vector space is the arbitrary choice of which ordered bases are "positively" oriented and which are "negatively" oriented. In the three-dimensional Euclidean space, right-handed bases are typically declared to be positively oriented, but the choice is arbitrary, as they may also be assigned a negative orientation. A vector space with an orientation selected is called an oriented vector space, while one not having an orientation selected, is called unoriented.

In mathematics, orientability is a broader notion that, in two dimensions, allows one to say when a cycle goes around clockwise or counterclockwise, and in three dimensions when a figure is left-handed or right-handed. In linear algebra over the real numbers, the notion of orientation makes sense in arbitrary finite dimension, and is a kind of asymmetry that makes a reflection impossible to replicate by means of a simple displacement. Thus, in three dimensions, it is impossible to make the left hand of a human figure into the right hand of the figure by applying a displacement alone, but it is possible to do so by reflecting the figure in a mirror. As a result, in the three-dimensional Euclidean space, the two possible basis orientations are called right-handed and left-handed (or right-chiral and left-chiral).